Slide Background

The Microbiology Department has been ISO certified since October 2022, approved as a Public Testing Laboratory by Food and drug Administration, Maharashtra since March 2010  for testing of cosmetics The Department is involved in Microbiological Testing of Cosmetic Raw materials and Finished Products.

Microbiology Laboratory equipped with a Bio-safety Unit, Scale-up and Testing activities for Cosmetics raw materials and Finished Good products.

Specialized Bioreactors for mass production of Microbial Cells.

Downstream Processing Section equipped for Extraction, Isolation, and Purification of Bioactive Components and analysis with GC, HPLC, HPTLC and GC/MS instruments.

Bio transformation of Organic Molecules.

In-vitro Antimicrobial Testing and the in-vivo Efficacy studies of actives and the final formulations used in cosmetics as per Standard BIS and ASTM Methods.

Testing activities in Microbiology Department

Sr. NoType of StudyReference Standard methodMethodType of samples Tested
1 Microbial examination of Cosmetics  As per BIS 14648-Total Viable count  (Bacterial and yeast & Mould count)
-Determination of Gram negative pathogens
E.g.:- Cosmetic raw material and finished products, soaps etc
2  Determination of Antimicrobial Activity by Serial Dilution MethodAs per BIS 11479Determination of effectiveness of antibacterial activity of soap samplesE.g.:- solid and liquid Soap samples
3Estimation of Minimum Inhibitory ConcentrationDetermination by Broth macro dilution methodE.g.:- Cosmetic raw material and finished products, soaps, sanitizers etc
4Determination of Antibacterial Activity  by Contact Kill MethodAs per BIS 11479Determination of Antibacterial activity by measuring its rate of kill against a selected organismE.g.:- Cosmetic raw material and finished products, soaps, sanitizers etc
5Determination of effectiveness of antimicrobial preservativesAs per  IP  /USP 51  Demonstration of the efficacy of the cosmetics preservative during the shelf and usage life of the cosmetic product. This is a regulatory requirement aimed at reducing risks to consumer safety.E.g.:- Cosmetic finished products
6Estimation of zone of InhibitionAs per BISBased on the principle of zone of inhibitionE.g.:- Cosmetic raw material and finished products, soaps, sanitizers, Deodarant, Anti dandruff and Anti acne activity
7Determination of Antimicrobial Activity By Time Kill Assay  ASTM E 2315Determination of antimicrobial activity by measurement of rate of kill of the organism by the formulation at regular time intervals. The percent kill is judged by comparing the kill of the Microorganism on contact with the sample to that with Solvent control.  E.g.:- Cosmetic raw material and finished products, soaps, sanitizers etc
8Evaluation of Antibacterial Products by Glove Juice method  ASTM E 1174Determination of effectiveness of antimicrobial handwashing agents for the reduction of transient microbial skin flora when used in a handwashing procedureE.g.:- Soaps, handwashes, sanitizers etc

Evaluation of antibacterial washes by Cup Scrub technique  ASTM 1874 & ASTM E 2752Evaluation of test materials containing antibacterial ingredients that are intended to reduce significantly the number of organisms on intact skin. It is also  used to provide an indication of residual antibacterial activity. The procedure is used in protocols intended to evaluate and identify resident flora.  E.g.:- Soaps, handwashes, sanitizers etc

Evaluation of effectiveness of SanitizersASTM 2755 Evaluation of healthcare personnel hand rubs/ Hand sanitizer against  microbesE.g.:- Sanitizers

Standard Cultures for Testing

Sr.No.Name of the organismSr.No.Name of the organism
1.Aspergillus brasiliensis ATCC 164047.Salmonella abony NCTC 6017
2.Candida albicans ATCC 10231  8.Staphylococcus aureusATCC 6538 
3.  Corynebacterium xerosis ATCC 3739.Staphylococcus aureus ATCC 6538P 
4.Escherichia coli ATCC 8739  10.Streptococcus mutans ATCC 25175 
5. Malassezia furfur ATCC 1452111.Staphylococcus epidermidis ATCC 49134
6.Pseudomonas aeruginosa ATCC 902712.Propionibacterium acne ATCC 11827